Electronic Hawk-eye technology is to be used to judge line calls at the Nasdaq 100 tournament later the month.
Similar technology has been used in other sport, but the Miami tournament will be its debut in a tour event.
"This is one of the most exciting things to happen in my 20 years in tennis," said Andre Agassi.
This year's US Open will be the first Grand Slam event to feature the system but individual tournaments can choose whether or not to use it.
The technology will be used on both the men's and women's tour, and it has received a warm welcome from players.
"As a player, I want to know that line calls are as accurate as technology will allow," said Maria Sharapova.
"In that sense, this announcement is great news for all players."
"This is perhaps the most significant change to the game since the tiebreaker", said the US Tennis Association's Arlen Kantarian.
Under the rules of the experiment, each player is entitled to challenge two calls per set. These will be reviewed instantly on a big screen.
The International Tennis Federation approved the technology last year and it was first tried out out at the end-of-season Champions Tour's event at the Royal Albert Hall.
In December, it received its first exposure at an elite event, in a Hopman Cup tie in Australia.
Source BBC Tennis